Journal: Nature chemical biology

Article Title: Sigma-1 receptor ligands control a switch between passive and active threat responses

doi: 10.1038/nchembio.2089

Figure Lengend Snippet: (a) Chemical structure of known sigma-1 ligands: (+)-SKF-10,047 ( 28 ), cutamesine ( 29 ), phencyclidine ( 30 ), noscapine ( 31 ), eliprodil ( 32 ) and donepezil ( 33 ). (b) Dose curves showing the degree of behavioral switching, measured by the freezing index. Data represent mean ± s.d. for n = 12 wells per dose and are representative of three independent experiments. (c) Full-range boxplots showing all data from n = 12 wells of wild-type sibling (WT) or homozygous mutant sigmar1 fish (8 or +25) treated with 10 μM scopolamine and either DMSO (−), 5 μM finazine or 10 μM cutamesine. Boxes represent the interquartile range, whiskers represent maximum and minimum values. Data are representative of two independent experiments. (d) Same as (c), but with wild-type sibling (WT) or homozygous mutant pgrmc1 fish (+8). Statistical significance was calculated using a student’s t -test (2-tailed, unpaired, unequal variance).

Article Snippet: The following compounds were re-ordered and used in all confirmatory and further assays: 1 finazine (ChemBridge, 6557321, ≥95%), 2 (ChemBridge, 6531872, ≥95%), 3 (ChemBridge, 6558983, ≥95%), 4 (ChemBridge, 6548654, ≥95%), 5 (ChemBridge, 6544610, ≥95%), 6 (ChemBridge, 6518299, ≥95%), 7 (ChemBridge, 6524759, ≥95%), 8 (ChemBridge, 6498979, ≥95%), 9 (ChemBridge, 6553891, ≥95%), 10 (ChemBridge, 6527322, ≥95%), 11 (ChemBridge, 6541071, ≥95%), 12 (ChemBridge, 7021430, ≥95%), 13 (ChemBridge, 7318202, ≥95%), 14 (ChemBridge, 7004866, ≥95%), 15 (ChemBridge, 7317879, ≥95%), 16 (ChemBridge, 7317926, ≥95%), 17 (ChemBridge, 7323150, ≥95%), 18 (ChemBridge, 6944181, ≥95%), 19 (ChemBridge, 6942573, ≥95%), 20 (ChemBridge, 5767877, ≥95%), 21 (ChemBridge, 6238692, ≥95%), 23 (ChemBridge, 6520799, ≥95%), 24 (ChemBridge, 6500628, ≥95%), 25 (ChemBridge, 6546312, ≥95%), 26 (ChemBridge, 9045050, ≥95%), naftopidil (Tocris, 0597, ≥98%), (+)-SKF-10,047 (Tocris, 1097, ≥98%), cutamesine (SA 4503, Tocris, 4951, ≥98%), phencyclidine (Sigma, P3029, ≥98%), noscapine (Tocris, 1697, ≥99%), eliprodil (Tocris, 2195, ≥98%), donepezil (Tocris, 4385, ≥99%) and scopolamine (Tocris, 1414, ≥99%).

Techniques: Mutagenesis

Journal: Journal of Neuroscience

Article Title: Impaired Attention in the 3xTgAD Mouse Model of Alzheimer's Disease: Rescue by Donepezil (Aricept)

doi: 10.1523/jneurosci.5242-10.2011

Figure Lengend Snippet: Figure 6. Effect of donepezil. a–d, Mean choice accuracy (a), omissions (b), perseverative responsesafteracorrectchoice(c),andperseverativeresponsesafteranincorrectchoice(d)of wild-type and 3xTgAD mice (n 8 per genotype, age 42–44 weeks) 1 h after saline, 0.03 mg/kg donepezil, 0.01 mg/kg donepezil, and 0.03 mg/kg donepezil injections, at a stimulus duration of 0.8 s. *p 0.05, simple main effect of genotype.

Article Snippet: Donepezil hydrochloride (Toronto Research Chemicals) was dissolved freshly each day in physiological saline (0.9% sodium chloride, pH 7.0; Aquapharm; Animalcare UK).

Techniques: Saline

Journal: Journal of Neuroscience

Article Title: Impaired Attention in the 3xTgAD Mouse Model of Alzheimer's Disease: Rescue by Donepezil (Aricept)

doi: 10.1523/jneurosci.5242-10.2011

Figure Lengend Snippet: Figure7. Effectofdonepezilonvigilance.a,b,Meanresponseaccuracies(a)andomissions (b) of wild-type and 3xTgAD mice (n 8 per genotype, age 42–44 weeks) after saline or donepezil treatment (pooled data of 0.03 mg/kg donepezil and 0.01 mg/kg donepezil). *p 0.05, simple main effect of donepezil.

Article Snippet: Donepezil hydrochloride (Toronto Research Chemicals) was dissolved freshly each day in physiological saline (0.9% sodium chloride, pH 7.0; Aquapharm; Animalcare UK).

Techniques: Saline

Journal: Iranian Journal of Pharmaceutical Research : IJPR

Article Title: High-Performance Liquid Chromatographic Determination of Rivastigmine in Human Plasma for Application in Pharmacokinetic Studies

doi:

Figure Lengend Snippet: The retention of rivastigmine, zolpidem, donepezil and norverapamil on different chromatographic columns

Article Snippet: Reagents Rivastigmine hydrogen tartrate and donepezil hydrochloride were obtained from Vasudha Pharma Chemicals Ltd. (Hyderabad, India).

Techniques:

Journal: Acta Pharmacologica Sinica

Article Title: Acetylcholinesterase-independent protective effects of huperzine A against iron overload-induced oxidative damage and aberrant iron metabolism signaling in rat cortical neurons

doi: 10.1038/aps.2016.78

Figure Lengend Snippet: A hypothetical scheme for the mechanism of HupA protection against iron overload induced neurotoxicity. Excessive iron treatment increases the level of LIP, in turn stimulates ROS generation and causes mitochondria dysfunction, which induces more ROS formation and eventually causes neuronal death. HupA significantly reduces the level of LIP and cellular ROS formation and ameliorates the mitochondrial function, and improves the cell viability. Interestingly, the mechanism of HupA protecting against iron overload neurotoxicity may not relevant to its cholinergic effect, in respect that inhibition of AChE activity by another specific AChE inhibitor–Don, to the same extent as that of HupA treatment, has no influence on iron overload induced LIP enhancement, ROS generation and mitochondrial dysfunction. HupA, huperzine A; Don, donepezil.

Article Snippet: Stock solutions of ferric ammonium citrate (FAC, Sigma-Aldrich, St Louis, MO, USA), deferoxamine (Sigma-Aldrich, St Louis, MO, USA) and donepezil hydrochloride (King-Pharm, Nanjing, China) were prepared in deionized water at 10 mmol/L.

Techniques: Inhibition, Activity Assay